dopamine receptor d1 drd1 Search Results


rabbit polyclonal d 1  (Alomone Labs)
94
Alomone Labs rabbit polyclonal d 1
Rabbit Polyclonal D 1, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mmp 2  (Proteintech)
93
Proteintech mmp 2
Mmp 2, supplied by Proteintech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dopamine receptor d1  (OriGene)
90
OriGene dopamine receptor d1
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Dopamine Receptor D1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dopamine receptor d1 drd1  (OriGene)
90
OriGene human dopamine receptor d1 drd1
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Human Dopamine Receptor D1 Drd1, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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d1r  (Alomone Labs)
90
Alomone Labs d1r
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
D1r, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dopamine receptor d1 (drd1) rs4532  (Tsang MD Inc)
90
Tsang MD Inc dopamine receptor d1 (drd1) rs4532
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Dopamine Receptor D1 (Drd1) Rs4532, supplied by Tsang MD Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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drd1 dopamine receptor d1  (Biotechnology Information)
90
Biotechnology Information drd1 dopamine receptor d1
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Drd1 Dopamine Receptor D1, supplied by Biotechnology Information, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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rat dopamine receptor d1 (drd1) elisa kit  (Reddot Biotech)
90
Reddot Biotech rat dopamine receptor d1 (drd1) elisa kit
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Rat Dopamine Receptor D1 (Drd1) Elisa Kit, supplied by Reddot Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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human dopamine receptor d1 (drd1)  (Vascular Dynamics)
90
Vascular Dynamics human dopamine receptor d1 (drd1)
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Human Dopamine Receptor D1 (Drd1), supplied by Vascular Dynamics, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dopamine receptor d1 (drd1) rabbit polyclonal antibody  (OriGene)
90
OriGene dopamine receptor d1 (drd1) rabbit polyclonal antibody
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Dopamine Receptor D1 (Drd1) Rabbit Polyclonal Antibody, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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dopamine receptor d1 (drd1) (nm_000794) human untagged clone  (OriGene)
90
OriGene dopamine receptor d1 (drd1) (nm_000794) human untagged clone
In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human <t>DRD1</t> (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor <t>D1;</t> FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.
Dopamine Receptor D1 (Drd1) (Nm 000794) Human Untagged Clone, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human DRD1 (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor D1; FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.

Journal: American Journal of Physiology - Renal Physiology

Article Title: G protein-coupled receptor 37L1 regulates renal sodium transport and blood pressure

doi: 10.1152/ajprenal.00289.2018

Figure Lengend Snippet: In human renal proximal tubule cells (hRPTCs), overexpression of GPR37L1 increases while silencing of GPR37L1 decreases intracellular sodium. hRPTCs were cultured in 12-well Transwell plates and transfected with the indicated plasmids. Two days later, the cells were serum starved overnight and treated with the indicated drugs at the luminal side for 30 min before loading with the sodium fluorescent dye. A: hRPTCs were transfected with control plasmid (empty vector, pcDNA), plasmid carrying cDNA encoding GPR37L1 (pCMV-GPR37L1), luciferase (LUC), β-galactosidase (GAL), or human DRD1 (pCMV-DRD1). n = 3/group; *P < 0.05 GPR37L1 vs. the rest, **P < 0.05 DRD1 vs. pcDNA. B: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with vehicle (Veh), nonselective NHE isoform inhibitor 5-(N-ethyl-N-isopropyl) amiloride (EIPA, 10 μM), or NHE3-selective inhibitor 3-[2-(3-guanidino-2-methyl-3-oxopropenyl)-5-methyl-phenyl]-N-isopropylidene-2-methyl-acrylamide dihydrochloride (S3226, 10 µM). n = 3/group; +P < 0.05 vs. pcDNA-Veh; n = 3/group. C: hRPTCs transfected with nonsilencing shRNA (NS-shRNA) or GPR37L1-shRNA (37L1-shRNA) and treated with Veh or S3226 at the luminal side similar to B. n = 3/group; ++P < 0.05 vs. NS-shRNA-Veh. D: hRPTCs transfected with plasmids pcDNA or pCMV-GPR37L1 were treated at the luminal membrane side with Veh, INM, PMA, FSK, or S3226. n = 3/group; $P < 0.05 vs. pcDNA-Veh, #P < 0.05 vs. GPR37L1-Veh. DRD1, dopamine receptor D1; FSK, forskolin; GPR37L1, G protein-coupled receptor 37L1; INM, ionophore ionomycin; NHE, Na+/H+ exchanger; CMV, cytomegalovirus.

Article Snippet: A plasmid encoding human GPR37L1 (pCMV- GPR37L1 ]) or dopamine receptor D1 (pCMV- DRD1 ), under the control of cytomegalovirus promoter, was obtained from Origene Technology (Rockville, MD; cat. no. GPR37L1 : SC117166; DRD1 : RC210389).

Techniques: Over Expression, Cell Culture, Transfection, Plasmid Preparation, Luciferase, shRNA